this post was submitted on 09 Jul 2023
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Houseplants

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Ignore the chaos in the background. ๐Ÿ˜… My dog came through and knocked everything over with the wiggles.

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[โ€“] [email protected] 1 points 1 year ago* (last edited 1 year ago) (1 children)

๐Ÿ˜ฑ Cool! This smells like the start of a new hobby....

[โ€“] [email protected] 2 points 1 year ago (1 children)

Haha good luck! I do tissue culture for a living. You definitely can do it at home but you have a lot of work cut out for you

[โ€“] [email protected] 1 points 1 year ago (1 children)

Haha, thanks! I have a few years of experience with home mycology (agar work/sterile technique) and I also keep lots of houseplants, so I'm thinking some of these skills might transfer when I do get into it. A reason why I'm interested in this is because some of the work I've read about fungi-root interactions was performed on fungi grown on root tissue cultures - which makes sense - but I don't really know how one would practically achieve that. So I'm very curious about it...

[โ€“] [email protected] 1 points 1 year ago (1 children)

Gotcha. While never having cultured fungus (on purpose) plants definitely pose unique challenges. Such as hormone application. I also think, though I don't have extensive knowledge and fungal media composition, that the media for plants can need quite a lot more sugar which makes bacteria and fungi even more a problem.

Really cool you've already done it at home though. Did you do pressure cooker method

[โ€“] [email protected] 2 points 1 year ago (1 children)

Such as hormone application.

Oh, I haven't worked with hormones before. That would be interesting.

Did you do pressure cooker method

Yeah, my pressure cookers are basically my autoclaves. And I use a still-air box (30L transparent plastic tub with holes) for pouring plates and transfers.

[โ€“] [email protected] 2 points 1 year ago

Oh yeah the hormones are cool you can make them do some weird stuff haha