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Does having too many pickups affect guitar tone, or sustain? Let's suppose a guitar has eight pickups while another one has just one of the same type. Would there be a tone difference, if the mega-pickup one was only using one of its pickups in a similar position to the single pickup guitar? Or would the effect of the extra pickups be too small to notice?
So, a pickup creates a magnetic pull on the strings, and this does have an effect on the way the strings are vibrating. It's an experiment you can do yourself: You can adjust the height of the pickup so that it gets closer to the strings. If it's close enough, you will hear a vibrato-effect when you're playing. Well, that's undesirable, unless you want to have vibrato everywhere.
So, if there's more magnetic pull on the strings, there's a higher possibility for unwanted vibrato; this could mean that you need to adjust the height, so that the pickups are lower (further away from the strings). Lower means a weaker signal/output; and the different strings might be affected differently, so this means different tone overall.
I have a guitar with a truckload of pickups. But I've never detected any vibrato - where the pitch oscillates up and down. Maybe this effect is not noticeable if the pickups are at a typical distance from the strings.