Science Memes

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Miracle cures (mander.xyz)

submitted 5 months ago by [email protected] to c/[email protected]

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[–] [email protected] 1 points 5 months ago (1 children)

jeez dude are you using some pretreatment

[–] [email protected] 1 points 5 months ago (1 children)

Idk what pretreatment really entails, but we did dilute it, if that counts 😅

[–] [email protected] 2 points 5 months ago

All HPLC samples that i do, before putting them on column i filter them through short layer of silica to separate all the insoluble and entirely too polar crap

Additionally there's also short guard column before main column, so if something goes really fucking wrong it's the 2cm guard column that is to be replaced and not the 20cm main one

I work mostly with NP-HPLC so your situation might be a bit different, but the general principle is there